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Mesophilic biohydrogen production by Clostridium butyricum CWBI1009 in trickling biofilter reactor

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dc.contributor.author Rathnasiri, PG
dc.contributor.author Laurent, B
dc.contributor.author Frank, D
dc.contributor.author Grigorescu, AS
dc.contributor.author Thonarta, P
dc.contributor.author Hiligsmann, S
dc.date.accessioned 2019-08-08T08:13:35Z
dc.date.available 2019-08-08T08:13:35Z
dc.identifier.uri http://dl.lib.mrt.ac.lk/handle/123/14739
dc.description.abstract This study investigates the mesophilic biohydrogen production from glucose using a strictly anaerobic strain, Clostridium butyricum CWBI1009, immobilized in a trickling bed sequenced batch reactor (TBSBR) packed with a Lantec HD Q-PAC5 packing material (132 ftVft3 specific surface). The reactor was operated for 62 days. The main parameters measured here were hydrogen composition, hydrogen production rate and soluble metabolic products. pH, temperature, recirculation flow rate and inlet glucose concentration at 10 g/L were the controlled parameters. The maximum specific hydrogen production rate and the hydrogen yield found from this study were 146 mmol H-/L.d and 1.67 mol Hj/mol glucose. The maximum hydrogen composition was 83%. Following a thermal treatment, the culture was active without adding fresh inoculum in the subsequent feeding and both the hydrogen yield and the hydrogen production rate were improved. For all sequences, the soluble metabolites were dominated by the presence of butyric and acetic acids compared to other volatile fatty acids. The results from the standard biohydrogen production (BHP) test which was conducted using samples from TBSBR as inoculum confirmed that the culture generated more biogas and hydrogen compared to the pure strain of C. butyricum CWBU009. The effect of biofilm activity was studied by completely removing (100%) the mixed liquid and by adding fresh medium with glucose. For three subsequent sequences, similar results were recorded as in the previous sequences with 40% removal of spent medium. The TBSBR biofilm density varied from top to bottom in the packing bed and the highest biofilm density was found at the bottom plates. Moreover, no clogging was evidenced in this packing material, which is characterized by a relatively high specific surface area. Following a PCA test, contaminants of the Bacillus genus were isolated and a standard BHP test was conducted, resulting in no hydrogen production. en_US
dc.language.iso en en_US
dc.subject Mesophilic Biohydrogen Trickling biofilter Immobilization Clostridium butyricum en_US
dc.title Mesophilic biohydrogen production by Clostridium butyricum CWBI1009 in trickling biofilter reactor en_US
dc.type Article-Abstract en_US
dc.identifier.year 2014 en_US
dc.identifier.journal International Journal of Hydrogen energy en_US
dc.identifier.volume vol. 39 en_US
dc.identifier.pgnos pp. 16902 - 16913 en_US


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